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ARTICLE
Year : 2011  |  Volume : 34  |  Issue : 4  |  Page : 221-224

Assessment of electron and gamma-induced dna damage in human peripheral blood by alkaline comet assay


1 Department of Studies in Physics, Mangalore University, Mangalagangotri, RPAD, India
2 Bhabha Atomic Research Centre (BARC), Mumbai, India

Correspondence Address:
Narayana Yerol
Department of Studies in Physics, Mangalore University, Mangalagangotri, RPAD
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0972-0464.106072

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In the present study, the effect of electron and gamma irradiation on the induction of DNA damage in human peripheral blood cells was investigated using comet assay. Blood samples were irradiated with an 8 MeV pulsed electron beam at a dose rate of 100 Gy min -1 . Gamma irradiation was carried out at a dose rate of 2 Gy min -1 using 60 Co gamma source. The total dose delivered to the samples was varied from 0 to 4 Gy. Samples were maintained at 0° C before irradiation, and the comet assay was carried out immediately after irradiation. Electrophoresis was performed at a field strength of 0.74 V cm -1 for 25 min at 4°C. A dose-dependent increase in DNA damage was observed. From the observed DNA damage, the relative biological effectiveness (RBE) for electron radiation with reference to gamma radiation on induction of DNA damage has been calculated.


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