Radiation Protection and Environment

ORIGINAL ARTICLE
Year
: 2013  |  Volume : 36  |  Issue : 4  |  Page : 168--174

Antioxidant, antibacterial, and ultraviolet-protective properties of carotenoids isolated from Micrococcus spp.


Devihalli Chikkaiah Mohana, Sreerangegowda Thippeswamy, Rayasandra Umesh Abhishek 
 Department of Microbiology and Biotechnology, Jnana Bharathi Campus, Bangalore University, Bengaluru, Karnataka, India

Correspondence Address:
Devihalli Chikkaiah Mohana
Department of Microbiology and Biotechnology, Jnana Bharathi Campus, Bangalore University, Bengaluru - 560 056, Karnataka
India

Carotenoids are the most common naturally occurring bioactive terpenoid pigments, which are commonly produced by a wide variety of plants and microbes. The present study was aimed to evaluate the antioxidant, antimicrobial and radio-protective properties of carotenoid pigments isolated from ultraviolet (UV)-C resistant Micrococcus spp. The UV-C resistant Micrococcus roseus and Micrococcus luteus were isolated from the soil samples of Savandurga hills region, Karnataka (India), and their pigments were identified as carotenoids based on spectral analysis. The UV-protective efficacies were determined by cling-film assay. Further, the antioxidant activities of pigments were evaluated by 2,2-diphenyl-1-picrylhydrazyl assay, and antibacterial activities by disc diffusion and broth microdilution assays. The optimum growth and pigment production by M. roseus and M. luteus were observed at temperature ranged between 35°C and 37°C, pH 7.0-8.0, NaCl 5.0-7.0%, and sucrose as major carbon and KNO 3 as major nitrogen sources. In the present investigation, the isolated carotenoid pigments of M. roseus and M. luteus showed significant UV protective activity along with antioxidant (IC 50 3.5-4.5 mg/mL) and antibacterial (minimal inhibitory concentration 0.25-2.0 mg/mL) properties.


How to cite this article:
Mohana DC, Thippeswamy S, Abhishek RU. Antioxidant, antibacterial, and ultraviolet-protective properties of carotenoids isolated from Micrococcus spp. Radiat Prot Environ 2013;36:168-174


How to cite this URL:
Mohana DC, Thippeswamy S, Abhishek RU. Antioxidant, antibacterial, and ultraviolet-protective properties of carotenoids isolated from Micrococcus spp. Radiat Prot Environ [serial online] 2013 [cited 2020 Nov 28 ];36:168-174
Available from: https://www.rpe.org.in/article.asp?issn=0972-0464;year=2013;volume=36;issue=4;spage=168;epage=174;aulast=Mohana;type=0